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KMID : 0880220080460050513
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Journal of Microbiology
2008 Volume.46 No. 5 p.513 ~ p.518
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Taxonomic Revision of the Nematode-Trapping Fungus Arthrobotrys multisecundaria
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Li Juan
Zhang Ke-Qin
Yang Jinkui
Liang Lianming
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Abstract
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The gene encoding an extracellular serine protease was cloned from Arthrobotrys multisecundaria using degenerate primers. The gene was highly similar (99.26%) to protease Mlx from Monacrosporium microscaphoides. To clarify the taxonomic relationship between these species, genes encoding the internal transcribed spacer (ITS) and β-tubulin were also cloned and sequenced from A. multisecundaria and M. microscaphoides, respectively. Homologous analysis of the nuclear (ITS) and protein (β-tubulin) encoding genes showed that the two species of nematode-trapping fungi also shared extensive identity (99.82 and 99.63%, respectively), although they exhibited obvious differences in secondary conidia morphology. Accordingly, a taxonomic revision is recommended, with A. multisecundaria being revised as A. microscaphoides var. multisecundaria. In addition, the identified mutation may better facilitate the study of the sporulation of nematode-trapping fungi.
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KEYWORD
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Arthrobotrys multisecundaria, Monacrosporium microscaphoides, serine protease, ITS, β-tubulin, taxonomy
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